Vacuoles and autophagosome-like structures in the degenerating epithelial glia.

<p>(A-E) Horizontal sections of the adult head lamina cartridge. (A) In <i>repo>GFP</i>.<i>nls</i> flies, two lamina neurons L1, L2 axons (L) and R1-6 axons (blue area) were surrounded by the electron-dense cytoplasm of the epithelial glia (G). The section was examined at three different depths, and the size of a single cartridge is not significantly different at different depths. (B-D) <i>repo>Shi</i>^<i>ts1</i> adults maintained at 29°C for 4 days. Axons were enlarged but intact. A large number of small and medium vacuoles were identified in the epithelial glia. (C) Small vacuoles in the glia that contained a double-membrane structure (arrowhead). (C’) Higher magnification of the boxed area in (C). (D) A double-membrane autophagosome-like structure (arrowhead) in the glial cytoplasm. (E) In <i>repo</i>^<i>ts</i><i>>DER</i>^<i>DN</i> adults maintained at 29°C for 2 days, large vacuoles were identified in between the lamina cartridges. N: glial nuclei. (E’) Higher magnification of the boxed area in (E), which shows autophagosome-like vesicles (arrowhead) in the glia cytoplasm.