Structural comparison of 14-3-3ζ-bound Cby with other 14-3-3 binding motifs comprising various +2 residues.

<p>Residues K49 and R56 are coloured yellow on the white surface representation of 14-3-3ζ. Structural and sequence alignment of Cby (green sticks) with the binding-motifs of (A). Raf1 (pink sticks, PDB: 3CU8), (B) PKCε (orange sticks, PDB: 2WH0), (C) Histone H3 (white sticks, PDB: 2C1N), (D) β2 integrin (blue sticks, PDB: 2V7D) and (E) α 4 integrin (purple sticks. PDB: 4HKC). The Cα RMSD values were computed by subtracting a Cα distance matrix between the -2 and +1 residues of Cby and each peptide as well as the -2 and most C-terminal residue in the respective alignments.