FloA and FloT distribute differently within the bacterial microdomains.

<p><b>(A and B)</b> PALM images of cells labeled with FloA-mEoS2 (A) or FloT-mEoS2 (B) translational fusions grown to stationary phase and fixed with PFA (4%). With increasing localization density the color code changes from red to yellow. White arrows indicate the localization of a cluster. Scale bars are 500 nm. Detail of the right bottom of each panel shows a dashed-line decorated PALM picture as a general indicator of the cell outline. Scale bar is 500 nm. <b>(C)</b> Comparative graph of the diameter of the clusters that were generated by FloA-mEoS2 and FloT-mEoS2 fluorescence signal. The upper right corner shows a graph with the mean of the diameter of the FloA and FloT clusters. <b>(D)</b> Comparative graph of the number of clusters detected in FloA-mEoS2 and FloT-mEoS2 labeled cells. <b>(E)</b> Comparative graph of the number of FloA and FloT localizations per cluster. <b>(F)</b> Comparative graph of the percentage of localizations that organized in clusters.