PECAM1 and β-catenin distribution pattern in 3 and 6 hours cultured 12.5 dpc male UGRs.

<p>Confocal microscopy analysis of PECAM1 distribution, observed by whole mount immunofluorescence in 12.5 dpc male gonads cultured for 3 (A-D) and 6 (E-H) hours in medium alone (A and E), and in the presence of RSPO1 (C and G). The corresponding TO-PRO3 staining (nuclei) are reported in B,D,F, and H. Arrowheads indicate the coelomic vessel. Confocal microscopy analysis of β-catenin distribution, observed by whole mount immunofluorescence in 12.5 dpc male gonads cultured for 3 (I-N) and 6 (O-R) hours in medium alone (I and L, O and P), and in the presence of RSPO1 (M and N, Q and R). All the images are merged with the respective TO-PRO3 staining. I,M,O, and Q show representative optical sections withdrawn at the organ surface while L, N, P, and R show representative optical sections withdrawn in the inner part of the same organs. ce: coelomic epithelium; tc: testicular cords. White arrowheads indicate β-catenin nuclear staining.