Kremen1 distribution pattern in 12.5 and 13.5 dpc male gonads.

<p>In panel A confocal microscopy analysis of Kremen1 distribution pattern, observed by whole mount immunofluorescence in 12.5 and 13.5 dpc male UGRs is reported. Kremen1 signal (FITC) detectable on the celomic surface of 12.5 and 13.5 dpc is shown in (I) and (V) respectively; the signal detectable in the inner part of 12.5 and 13.5 dpc male gonad is reported in (II) and (VI); the corresponding TO-PRO3 staining (nuclei) is reported in (III) and (VII) and the corresponding bright fields are shown in (IV) and (VIII). In panel B a higher magnification of 13.5 dpc testis Kremen1 immunofluorescence is reported. The signal detectable in the coelomic portion of the gonad is shown in (I) whereas the signal in the inner part of the testis is shown in (IV); the corresponding TO-PRO3 staining (nuclei) is reported in (II) and (V) and the corresponding bright fields are shown in (III) and (VI). ce: celomic epithelium; ms: mesonephros; tc: testicular cords; ts: testis.