LGR4 distribution pattern in 12.5 and 13.5 dpc male gonads.

<p>Confocal microscopy analysis of LGR4 localization, observed by whole mount immunofluorescence in 12.5 and 13.5 dpc male UGRs. In the left part of the panel LGR4 signal (FITC) and PECAM1 signal (Cy5) on the coelomic surface (A and B) and in the inner part (E and F) of 12.5 dpc male gonad are reported; images C and G show the merging images of FITC and Cy5 signals while D and H show the corresponding bright fields. In the right part of the panel, LGR4 signal (FITC) and PECAM1 signal (Cy5) on the coelomic portion (I and L) and in the inner part (O and P) of 13.5 dpc male gonad are reported; images M and Q show the merging images of FITC and Cy5 signals while N and R show the corresponding bright fields. White arrowheads indicate LGR4 positive endothelial cells. ce: coelomic epithelium; tc: testicular cords.