Effect of the Cy3_ HP_ LNA/2OMe _PS oligonucleotide probe on viability (MTS assay, A) and cell death (apoptosis, B and C) of AGS cells.

<p>AGS cells were treated with 200 nM of Cy3_ HP_ LNA/2OMe _PS oligonucleotide probe for 24h. The results are the mean ± SEM of three independent experiments; *<i>p<0</i>.<i>05 vs</i> untreated cells by ANOVA. A) Cell viability was measured using MTS assay. B) Analysis of caspase-3/7 activity in AGS cells treated with Cy3_ HP_ LNA/2OMe _PS oligonucleotide probe. Staurosporine, STS, was a positive control of cell death. C) Effect of Cy3_ HP_ LNA/2OMe _PS oligonucleotide probe on the amount of DNA fragmentation in the cultured gastric cell line AGS. The level of apoptosis occurring with each treatment was determined by cell death ELISA^Plus kit.