Gene expression during immune response of <i>w</i> larvae measured by q-PCR.

<p>(A) Reciprocal changes in mRNA expression of glycogen synthase and glycogen phosphorylase enzymes in the fat body. (B) Summary of significant changes in expression of glycolytic and citrate cycle enzymes in the hemocytes, lymph gland, and fat body (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002135#pbio.1002135.s004" target="_blank">S3</a>–<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002135#pbio.1002135.s006" target="_blank">S5</a> Figs for corresponding graphs). Heat map indicates a tendency of glycolytic genes to increase in immune cells and to decrease in fat body. (C) Expression of trehalose transporter Tret1-1 in the fat body. (D) Expression of GLUT1, TreT1-1, and trehalase in the circulating hemocytes and lymph gland. All graphs except (B) show mean values of expression relative to <i>Rp49</i> ± SEM from three independent experiments; grey columns: control larvae, black columns: infected larvae; asterisks indicate significant changes (tested by two-way ANOVA).