Binding of GII.4 P particles to synthetic oligosaccharides.

<p>P particles from 11 strains of GII.4 viruses (2005Y2004C, 2006Y2004C, 2007Y2006bC, 2007Y2008bC, 2010Y2008bC, 2010Y2010C1, 2011Y2010C1, 2011Y2010C4, 2012Y2010C1, and 2012Y2010C2) were coated onto 96-well plates at 2 μg/ml, and then incubated with oligosaccharides (H type 1, H type 2, H type 3, A, B, Le^b, Le^y, Le^a, Le^x, Sialyl-Le^a, or Sialyl-Le^x) overnight. The y-axis indicates captured HBGAs detected by HRP-avidin (optical densities at 450 nm were averaged from at least two independent experiments), and the 98Y96C (VA387) P particle was set as the positive control for binding.