Sensitivity of the qPCR assay for detection of <i>V</i>. <i>cholerae</i> O1 El Tor N16961.
- Luigi Vezzulli (426915)
- Monica Stauder (730682)
- Chiara Grande (730683)
- Elisabetta Pezzati (426916)
- Hans M. Verheye (730684)
- Nicholas J. P. Owens (730685)
- Carla Pruzzo (426918)
Publication date
April 2015
DOIAbstract
<p>DNA was amplified with the gbpA TaqMan primers in the presence of the gbpA fluorogenic probe. (A) Amplification plot of <i>V</i>. <i>cholerae</i> ATCC 39315 sample dilutions containing 10<sup>6</sup>, 10<sup>5</sup>, 10<sup>4</sup>, 10<sup>3</sup>, 10<sup>2</sup>, 10<sup>1</sup> genome copies per reaction (GU/rx). (B) Plot of mean Cq-values from three replicates tested against the <i>V</i>. <i>cholerae</i> DNA inputs. Error bars indicate the standard deviations of the means.</p
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