<i>In vitro</i> BBB penetration assay.

<p>Analysis of the penetration ratio of different compounds using an <i>in vitro</i> model of BBB. A permeability coefficient (Papp) above 20 x 10^–6 cm/s is considered to provide a very good penetration into the CNS, while a Papp lower than 10 x 10^–6 cm/s is consistant with a low penetrant CNS drug. TM5484 has a 67.6 x 10^–6 cm/s permeability coefficient. Verapamil (N.D.) and Propanolol (31.3 x 10^–6 cm/s) were used as negative and positive control, respectively. Data are mean±SD. n = 3 for each compound. N.D. indicates not detectable.