STIM1 is enriched in patches at the <i>C</i>. <i>trachomatis</i> inclusion membrane.

<p>Confocal micrographs of HeLa cells infected with <i>C</i>. <i>trachomatis</i> for 24h and stained using antibodies against the <i>C</i>. <i>trachomatis</i> inclusion membrane protein IncA (A-B) (left panels, IncA, green (A) and red (B)). The cells were transfected with a mCherrySTIM1 construct 18h pre-infection (A) (middle panels, mChSTIM1, red) or stained using antibodies against STIM1 (B) (middle panels, STIM1, green). The merge is shown on the right. The top and bottom panels respectively correspond to the extended focus view combining all the confocal planes (Ext.Foc.) and a single plane crossing the middle of the inclusion (XY View). The asterisk in the XY View Merge panel indicates the inclusion. Scale bar: 10μm.