Cardiac specific activation of WDR12 by adenoviral gene delivery into the LV.

<p><b>A.</b> Different doses of WDR12 adenoviral constructs were tested to increase LV WDR12 protein levels. Representative Western blots are shown. Bands were detected from the same gel. The results are expressed as mean±SEM (n = 3). *<i>P</i><0.05, **<i>P</i><0.01 versus 1x10⁹ IFU/rat injected group (1-way ANOVA followed by LSD post hoc test). <b>B.</b> WDR12 mRNA levels measured by RT-PCR, and <b>C.</b> WDR12 protein levels assessed by Western Blot analyses from the tissue samples 3 days, 1 week and 2 weeks after WDR12 gene delivery. Bands were detected from the same gel. Open bars represent LacZ and solid bars WDR12. The results are expressed as mean±SEM (n = 8 to 10). *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001 versus LacZ (Student <i>t</i> test). <b>D.</b> Immunofluorescence staining of LV anterior wall at day 3 after gene transfer showing WDR12 predominantly in cardiomyocytes (WDR12, red).