Distinct pollen defects in <i>tun</i> and <i>evn</i> mutants.

<p>(A) Pollen in vitro germination assay of <i>qrt/qrt</i> pollen grains. (B) Pollen in vitro germination assay of <i>tun-1/TUN;qrt/qrt</i> pollen. Arrows indicate PT bursting. (C) Graph of PT bursting counts in <i>qrt/qrt</i> and <i>tun-1/TUN;qrt/qrt</i> pollen. (D) Pollen in vitro germination assay of <i>evn-1/EVN;qrt/qrt</i> mutant pollen. Arrowheads indicate degenerated pollen grains. (E) Graph of degenerating pollen grain counts at different stages of <i>evn-1/EVN</i> mutants after DAPI staining. Stage four refers to the bicellular and early tricellular, stage three to the tricellular, stage two to the late tricellular and early mature, and stage one to the mature pollen stage. Scale bars: 20 μm. (F–G) Gene model of <i>TUN</i> (F) and <i>EVN</i> (G) with mutant alleles. Ethane methyl sulfonate (EMS) single nucleotide polymorphisms (SNPs) are indicated by lines, T-DNA insertions by triangles.