Nuclear P-Smad 1/5/8 fluorescence in response to BMP2.

<p>Nuclear Phospho-Smad 1/5/8 staining in blastema mesenchymal cells in blastema slices cultured in the presence or absence of exogenous human BMP2 in amounts as indicated. The corrected nuclear fluorescence was calculated in order to normalize the intensity of nuclear staining for variation in the area of the nuclei being analyzed as well as for the background fluorescence [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0123186#pone.0123186.ref040" target="_blank">40</a>]. Error bars represent S.E.M., and P-values were determined by t-test with 2 tails assuming unequal variances.