RNA from Phenol-Chloroform Extract (PCE) and Qiagen column fraction (QRNA) from CD3+ CD8+ suppressor T lymphocyte (Ts) culture supernatant (Sup) is suppressive.

<p><u><b>a</b></u><b>.</b> PCE from TNP Ts Sup suppresses the adoptive transfer of CS-effector cells (Groups B and D). <u><b>b</b></u>. Treatment of Ts cells with anti-CD3 mAb plus complement (C’) eliminates production of suppressive Sup (Group D). <u><b>c</b></u><b>.</b> Similar treatment of Ts cells with anti-CD8 mAb plus C’ also eliminates generation of suppressive Sup (Group D), while anti-CD4 mAb plus C’ treatment does not (Group C). <u><b>d</b></u><b>.</b> QRNA from TNP Ts Sup PCE separated on a Qiagen column suppresses adoptively transferred CS, while the QDNA fraction is inactive (Group C vs D). <u><b>e</b></u><b>.</b> TNP- and OX-specific Ts Sup-derived PCE (Groups B and C) and QRNA (Groups E and F) inhibit HT-2 cell in vitro responsiveness to IL-2, compared to Nl Cell Sup PCE and QRNA (Groups A and D).