Effect of <i>PPA1</i> knockdown on the colony formation and cell viability in MCF7 cells.

<p>(A) Representative picture of colony formation assay in MCF7 cells. PPA1 shRNA and scramble shRNA transfected cells were seeded in 12 well plates and colonies were visualized by crystal violet staining after 2 weeks of puromycin selection. (B) Number of colonies of MCF7 cells transfected with scramble shRNA or, PPA1 shRNA from triplicate wells, p˂0.05. (C) Effect of PPA1 shRNA on the cell survival. MCF7 cells transfected with scramble shRNA or PPA1 shRNA were seeded in 24 well plates in triplicate and selected with puromycin. Cell viability was analyzed by the MTT assay at 24 h and 48 h of puromycin selection. Data represents mean ± S.E.M of two independent experiments, p˂0.05. (D) Western blot showing knockdown of PPA1 after 48 h of transfection of PPA1 shRNA in MCF7 cells.