PLGF protein and gene analysis showed up-regulation in ES treated samples.

<p>A. Immunohistochemical analysis of PLGF. PLGF was significantly up-regulated on days 3 and 7 of ES treated tissues compared to control of respective days. Even though ES treated tissues showed increase of 21% and 23% on days 10 and 14 respectively when compared to control, the results were not statistically significant (p>0.05). The arrows indicate PLGF⁺ cells. B. Western blotting of PLGF. Protein analysis by Western blotting showed significant increase of PLGF on days 7 and 14 in ES treated tissues compared to normal healing tissues. C. Gene analysis of VEGF-A on different healing days showed up-regulation in ES treated tissues on days 10 and 14, compared to control. Grey bars—Normal cutaneous healing process. Black bars—Electrical stimulation assisted cutaneous healing process. * shows significant difference (p<0.05).