VEGF-A protein and gene analysis showed up-regulation in ES treated samples.

<p>A. Immunohistochemical analysis of VEGF-A. VEGF-A was significantly up-regulated on days 7, 10 and 14 of ES treated tissues compared to normal healing tissues of respective days. Number of vessels were also increased on day 14 ES treated tissues compared to normal healing tissues. Arrows/area inside the dotted lines indicate VEGF⁺ cells/area. B. Western blotting of VEGF-A. Protein analysis by Western blotting showed increase on day 14 ES treated tissues compared to control. C. Gene analysis of VEGF-A on different healing days showed up-regulation in ES treated tissues, compared to control. Grey bars—Normal cutaneous healing process. Black bars—Electrical stimulation assisted cutaneous healing process. * shows significant difference (p<0.05).