Breadth and magnitude of the IgG response to <i>P</i>. <i>falciparum</i> antigens by HIV status.

<p>(A) A microarray containing 824 <i>P</i>. <i>falciparum</i> proteins or protein fragments was probed with plasma samples from HIV+ (n = 18) and HIV- (n = 18) adults during symptomatic malaria. A. Venn diagrams showing the number of reactive antigens among HIV+ subjects (orange), HIV- subjects (blue), both HIV+ and HIV- subjects (purple) or neither (254). (B) Antibody breadth of HIV+ individuals (mean 83 antigens) and HIV- individuals (mean 208 antigens). Mean values and standard deviations are shown; Significant differences in breadth (Negative Binomial generalized linear model) (C) Magnitude of <i>P</i>. <i>falciparum</i> IgG responses by HIV status. We examined 384 antigens that were recognized in ≥ 10% of all samples and show the average IgG reactivity of each by HIV status. IgG reactivity is significantly higher in HIV- group (blue bars) compared to HIV+ group (orange bars) for 173 antigens. The red horizontal line indicates a p value of 0.05. (Empirical Bayes Moderated t-test, p<0.05, and an absolute log fold change > 1).