EC50 profile of D9 in human cancer cell lines.

<p>(a) Chemical structure of D9. (b) Bar graphs showing EC50 of D9 measured in a panel of solid (bi) and blood (bii) cancer cell lines using cell viability assay.1 x 10^³ cells of individual cell lines were seeded into 96-well plates in triplicates and D9 at 10 different doses was added 24 hours post cell seeding. Proliferation was measured after 96 hours treatment of D9 using an ATP based cell viability assay. EC50 of D9 was calculated by nonlinear regression (curve fit) using GraphPad PRISM3. Data represent the means of EC50 of D9 measured in three independent experiments, with each experiment run in triplicates.