IFIT1 inhibits translation of incoming alphavirus-specific RNAs.

<p><b>(A)</b> Wild type (wt), IFIT1^-/-, IFIT2^-/- or IFIT locus^-/- MEF were treated for 20 h with the indicated concentrations of IFN-β or remained mock-treated. Then cells were infected with EIL/nLuc/VEEV at an MOI of 10 PFU/cell for 1 h at 37°C, washed with PBS, and further incubated in complete medium at 37°C. nLuc activity was analyzed at 4 h post infection, and data were normalized to samples from the mock-treated, infected cells. <b>(B)</b> Wt and IFIT1^-/- MEF were treated for 20 h with the indicated concentrations of IFN-β or remained mock-treated. Then cells were infected with EIL/5’wtVEEV-nLuc/VEEV and EIL/5’TCVEEV-nLuc/VEEV viruses. nLuc activity was analyzed at 4 h post infection and normalized to the activity in the samples of the mock-treated, infected cells. One of the reproducible repeated experiments is presented. All of the measurements were performed in triplicates, and standard deviations are too small to be seen.