Cell proliferation assay in the presence of conditioned media.

<p>RM1 cell proliferation cultured with different conditioned media, assessed by the percentage of tetrazolium salt reduction. RM1 proliferation cultured with preadipocyte conditioned media (PACM) compared with the control (p>0.05, Mann-whitney U test) (A). RM1 proliferation cultured with 24 and 48h adipocyte conditioned media (ACM) compared with the control (*** p<0.001 one-way ANOVA, Newman-Keuls multiple comparison post-hoc test) (B). RM1 Proliferation with three different conditioned media (* p<0.05 one-way ANOVA, Newman-Keuls multiple comparison post-hoc test) (C).