Invasion assay.

<p>Number of RM1 cells that invaded the matrigel membrane of the transwell system after 24 h in co-culture with preadipocytes, adipocytes and control media, used to evaluate invasion capacity, which was significantly higher in both co-cultures compared with control media alone (* p<0.05 Unpaired T test) (A). Representative images of the cells invading the matrigel membrane for each tested condition (B).