Effect of TRPC6 inhibitor on intracellular calcium in human platelets <i>in vitro</i> and on ERK and Akt phosphorylation.

<p>(A) Human platelets were loaded with Fura-2/AM to measure intracellular [Ca²⁺]_i, in the presence or absence of TRPC6 inhibitor (10 μM), and activated with U46619 (0.5 μM), ADPβS (100 μM) and OAG (150 μM). Each experiment was repeated at least three times, with blood obtained from three separate donors. (B) Human platelets were incubated in the presence or absence of TRPC6 inhibitor (10 μM) for 5 minutes and then stimulated with U46619 (0.5 μM), ADPβS (100 μM) for 3 minutes, and subjected to immunoblotting with ERK, pERK, Akt and pAkt antibodies.