Peptide loss during successive transfers of 250 μ L 5 μ M solutions between sample containers.

<p>The solutions were successively transferred between borosilicate glass vials, polypropylene tubes, or Protein LoBind tubes. The percentage of recovered peptide was measured for mastoparan X (<i>A</i>), melittin (<i>B</i>), and magainin 2 (<i>C</i>) solutions as a function of the number of sample containers in which the solutions had been incubated. In all panels, the data are the average of two separate experiments. The error bars show the standard deviations. The error bars are not shown if they are smaller than the symbols. The data show that peptide is dramatically lost when peptide solutions are successively transferred between borosilicate glass vials or polypropylene tubes.