Double immunofluorescence staining for phosphorylated SREBP-2 in HepG2 cells.

<p>HepG2 cells were treated with AICAR (1 mmol/L) for 12 h or TSH (4 μM) for 24 h. The yellow in the images is derived from dual staining with the red p-Thr antibody and the green anti-SREBP-2 antibody. The nuclei were detected with 4', 6-diamidino-2-phenylindole (DAPI). Original magnification, The intensity of staining obtained with SREBP-2/p-Thr was measured by ImageJ.