Increased B-ADAM10 and decreased ADAM17 and TNF in Th2 biased strains.

<p>Naïve B220⁺ B cells (A, B) stained for total ADAM10 (A) or ADAM17 (B) expression by flow cytometry. (C) TNF coexpression on 3 day stimulated (anti-CD40+IL4) B220⁺ B cells by TSA. (A,B,C) Bar graph represents percent total B cells staining high in ADAM10 (A), ADAM17 (B), or TNF (C). (A,B,C) Balb/c (──)A/J (——), C57Bl/6 (▬▬), SJL (▪▪▪▪▪), respective isotype control (shaded grey) in representative overlay (left); grey gate demonstrates high stained population. n = 8 per group, 3 independent studies. *p<0.05, **p<0.005, ***p<0.0005. (D) TNF message from 3 day stimulated (anti-CD40 + IL4) B220⁺ B cells. Presented as fold change from C57Bl/6 WT. * signifies ≥2 fold change between C57Bl/6 WT and the listed WT strain.