Effect of MT3 expression on breast cancer cells growth <i>in vitro</i> and tumorigenesis.

<p><b>(A)</b> Proliferation of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. Cell proliferation was determined <i>in vitro</i> using MTT assay, as described in the “Materials and Methods”. The values are shown as the mean ± SD of three independent experiments. <b>(B)</b> Xenograft tumor growth of control BO2/LUC/PURO cells and BO2/MT3/LUC/PURO cells overexpressing MT3. Tumor growth was recorded on a weekly basis using metric calipers. Data are shown as the mean tumor volume for each group of mice (n = 5) at each indicated time point. <b>(C)</b> Haematoxylin and eosin stained tumor sections presenting spindle-like cancer cells with visible mitotic figures and <b>(D)</b> fibrous capsule surrounding the tumor mass. Arrows indicate mitotic figures (<b>C</b> and <b>D</b>); arrowheads, fibrous capsule (<b>D</b>). Magnification ×200.