Loss of CD8+ T cell cytotoxicity abrogates the effects of picornavirus vaccination against GL261-Quad gliomas.

<p>(A) Mean bioluminescence intensity (p/sec) of wild type C57BL/6 or perforin deficient (<i>Prf</i>^<i>-/-</i><i>)</i> GL261 glioma-bearing mice demonstrates no difference in the rate of tumor progression in the absence of treatment (N = 18 mice/group). (B) Mean BLI (p/sec) and (C) survival curve of C57BL/6 (N = 10 mice/group) or <i>Prf</i>^<i>-/-</i> (N = 7 mice) GL261-Quad glioma-bearing mice following picornavirus vaccination demonstrates a complete loss of vaccine efficacy in the absence of functional cytotoxic CD8+ T cell activity. Error bars indicate SEM. * denotes p<0.05, ** denotes p<0.01, ***denotes p<0.001. Arrow denotes time of vaccine administration.