Flow cytometry of FoxP3⁺ CD4⁺ T cells and RORɣt+ CD4+ T cells.

<p>CD4^<b>+</b> T cells were isolated by CD4+ Tcell isolation kit II human of Miltenyi Biotec and stained with respective antibodies and evaluated in relapsing phase (n = 20) and remitting phase (n = 20) of MS patients and healthy controls (n = 10). A forward and side scatter gate was used to select lymphocyte population and fluorescence compensation was set according to labeled lymphocytes with only green and only red fluorescent separately versus isotype control (<i>A</i>). Percentage of RORγt+ CD4+ T cells measured by Flow cytometry, shows meaningful increase in relapsing group (<i>B</i>) while percentage of FoxP3^<b>+</b> CD4^<b>+</b> T cells elevates in remitting group (<i>C</i>) (*p < 0.05, **p < 0.01 and ***p < 0.005, non-parametric Mann-Whitney <i>t</i>-test) (RP: Relapsing patient, MP: Remitting patient, HV: Healthy volunteer).