Effect of milk diets on expression of tumor markers.

<p>Various tumor markers relevant to Pb-Prl (A) and KIMAP (B and C) models were assessed in DP following 27- or 15-week milk diets respectively, as compared to control (water) group. Proliferation index (Ki-67 staining, shown for both models), activated Stat5 (p-Stat5) and p63 expression (for Pb-Prl mice) and c-Fes expression (for KIMAP mice) were assessed from immunohistochemical (IHC) analysis (n = 6 per group; pictures show representative staining, scale stands for 50μm). For each marker (except c-Fes), IHC were quantified and the results are represented on adjacent bar histograms and are expressed as fold change <i>vs</i>. control (water) group. L, lumen of the glands. (C) q RT-PCR analysis of c-Fes, Ki-67 and Gprc6a mRNA expression in DP of KIMAP mice following 15-week milk diets. Results are expressed as fold change <i>vs</i>. control (water) group and are shown as means ± S.D. P values are represented <i>vs</i>. water control group (*). <i>nd</i>, for not determined.