CD133 identifies CSCs in colorectal cancer.

<p>(A)Schematic of CD133⁺ and CD133^-/lo tumor cells sorting from dissociated colorectal xenograft tumor by FACS. (B) A representative example of post-sorting analysis of the sorted CD133⁺ and CD133^-/lo XhCRC cells.(C) Tumor-initiating frequency of CD133⁺ and CD133^-/lo CRC cells in immunodeficient mice (D-G)Serial sphere formation assays for purified CD133⁺ and CD133^-/loCRC cells (i.e., XhCRC and SW620). Spheres were enumerated (D, F) and representative images are shown (E, G).Scale bars, 100μm.***<i>P</i>< 0.001.