HTT colocalizes with RAB11A and regulates RAB11A activity during apical vesicular trafficking.

<p>(A) Mammary gland section stained for HTT 4C8 and RAB11A. (B) Mammary gland section stained for E-cadherin and RAB11A. (C) Twenty-four–hour and four-day MDCK 3-D cultures stained for HTT 4C8 and RAB11A. Colocalization of HTT and RAB11A is displayed in yellow (merge). (D) Twenty-four–hour and four-day MDCK 3-D cultures stained for RAB11A. HTTFL is tagged with mCherry and fluorescence is displayed in magenta and the colocalization of RAB11A and HTTFL appears in white. (E) Twenty-four–hour and four-day MDCK 3-D cultures transfected with RAB11A^WT, RAB11A^Q70L or RAB11A^S22N, stained for PAR3. RAB11 is tagged with GFP and fluorescence is displayed in magenta. The colocalization of aPKC and RAB11A appears in white. (F) Representative line-scan analysis (relative fluorescence intensity; at least 20 cells were analyzed per condition). (G) Percentage of acini with normal lumen. (H) Quantification of acini size. (G and H) Control+RAB11A^WT: <i>n</i> = 59 acini, Control+RAB11A^Q70L: <i>n</i> = 54 acini, Control+RAB11A^S22N: <i>n</i> = 66 acini, shHTT2+RAB11A^WT: <i>n</i> = 60 acini, shHTT2+RAB11A^Q70L: <i>n</i> = 72 acini, shHTT2+RAB11A^S22N: <i>n</i> = 93 acini. (I) FM64-4 4-day MDCK 3-D structures were video recorded. Maximum intensity and z projections are shown. All scale bars, 10 μm. Error bars, SEM. ** <i>p</i><0.01; *** <i>p</i><0.001. Complete statistical analyses with number of measures are detailed in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002142#pbio.1002142.s001" target="_blank">S1 Data</a>.