Flagellin induces TNF-α expression in a time-dependent and dose-dependent manner.

<p>Ana-1 cells were treated with flagellin, and supernatants were assayed for TNF-α by ELISA. A. flagellin (100 ng/ml) for 0, 4, 8, 12, and 24 h. B. flagellin (0, 1, 10, 100 ng/ml) for 24 h. Experiments were made in triplicate, and data are representative of three separate experiments (mean ± SD).