Population sequencing reveals multi-step adaptation of residues in the DAF-specific footprint during passage in A549 cells.

<p>(A-F) The virus populations were purified and deep sequenced for each replicate every two passages (p1, 3, 5, 7, etc...) across the P1 region coding for the four capsid proteins. The variant frequencies were determined by the ViVAN pipeline and all statistically significant mutations that showed a trend of increasing frequency were plotted in linear scale (left panels) and log scale (right panels). Each set of linear and log graphs represents the same data from one replicate, all six replicate passage series presented in this work are shown.