Altering levels of drebrin and pS142 phosphorylation of drebrin affects neuroblast distribution in the OB.

<p>P2 mice were electroporated in the lateral ventricle with plasmids encoding YFP or YFP-tagged wt, S142A or S142D drebrin. Sagittal brain slices were prepared 14 days later and immunostained for YFP. (A, top row) Representative confocal projections of sagittal brain slices showing overall labeling in the OB and overlaid with the mask used for analysis (area A, inner OB; area B, outer OB). High magnification pictures of the OB insets are shown in the bottom row. (B) Schematic diagrams of a typical quantification analysis using all OB sections from one brain for each condition. Cell bodies in areas A and B are represented as dots. (C) Overexpression of wt, S142A and S142D drebrin caused a significant cell accumulation in the inner OB compared to GFP (mean ± SEM; n = 3 brains for each construct; *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001). Scale bars: (A, top row), 500 μm; (A, bottom row), 100 μm.