Altering levels of drebrin and pS142 phosphorylation affects neuroblast migration along the RMS.

<p>P2 mice were electroporated <i>in vivo</i> with plasmids encoding YFP, or YFP-tagged wt, S142A, or S142D drebrin. Fluorescently-labelled RMS neuroblasts were imaged 5 days later in acute brain slice cultures over a period of 3 hours. (A) Representative migration paths of neuroblasts expressing YFP empty vector, YFP-tagged wt, S142A, or S142D drebrin. Yellow asterisks mark the location of the OB. Quantitative tracking analysis reveals that neuroblasts overexpressing any one of the drebrin versions display a lower migratory distance (B), displacement (C), speed (D) and a trend towards a lower migratory index (E) compared to control cells expressing only YFP (mean ± SEM; n = 6 brains for control; n = 5 brains for wt; and n = 4 brains for S142A and n = 5 for S142D; **<i>P</i><0.01, ***<i>P</i><0.001).