Representative of regeneration in the distal end of the nerve and denervated muscle 2 months after nerve anastomosis.

<p>(A, B, C, D) Expression of neurofilament staining in the normal nerve and in the distal end of nerve subjected to different treatments. (E, F, G, H) Expression of S-100 staining in the normal nerve and in the distal end of nerve subjected to different treatments. (J, K, L, M) Expression of acetylcholine receptor staining in normal muscle and the denervated muscle subjected to different treatments. (N, O, P, Q) Expression of desmin staining in the normal muscle and the denervated muscle subjected to different treatments (I) Quantitative analysis of the density of neurofilament and S-100 in distal end of nerve subjected to different treatments (R) Quantitative analysis of the density of acetylcholine receptor and desmin in the denervated muscle subjected to different treatments. (S) The representative of western blot analysis in different treatment related to expression of desmin and Ach R (acetylcholine receptors). Bar length = 100 μm, N = 6 for each group, *p<0.05, **p<0.01 indicated the statistical difference as compared to the control group; # p<0.05, ##p<0.01 indicated the statistical difference as compared to the ES group.