Assessments specific for ribosomal RNAs before and after hyphal stimulation in 10% FBS in H₂O up to 60 minutes.

<p>A. RNA was gel separated and stained with SYBR Gold stain. Lane 1 has RNA size markers. 5μl of RNA were loaded in each lane as follows: Lane 2- prior to stimulation, Lane 3 after 30 min of stimulation, Lane 4 after 60 min stimulation. All three subunits are decreased by 30 min and remain so at 60 min. B. Quantitative PCR was performed on total RNA extracted before and after serum stimulation (see text). Bars represent ratios of amplicon numbers generated after stimulation to those generated prior to stimulation. Error bars represent SD. T = 0 time points were normalized to 1. Length of stimulation indicated under bars. Color scheme indicates primers specific for each ribosomal RNA sub-unit. All three subunits are decreased by 30 min and remain below baseline at 60 min.