Analysis of mitochondrial membrane potential (ΔΨ_m).

<p><i>T</i>. <i>brucei</i> bloodstream forms were incubated in the absence (light grey bars) or presence (dark grey bars) of tetracycline to down-regulate TbMCP14 by RNAi (panel A) or over-express TbMCP14 (panel B) for 3 days before treatment with G25. Parasites were treated with different concentrations of G25 for 24 h followed by addition of 25 nM TMRE for an additional 30 min and analysis by flow cytometry. Carbonyl cyanide <i>m</i>-chlorophenyl hydrazone (CCCP) was added to control cultures to disrupt ΔΨ_m. The values were normalized relative to untreated control trypanosomes. The data points represent mean values ± SD from at least four independent experiments. The asterisks represent statistical difference relative to the respective uninduced control (*p<0.05, **p<0.01, unpaired student’s t test).