Sequence analysis of ORF3a and flanking regions.

<p>Pseudo-alignment of representative luteovirus and polerovirus NCBI species RefSeqs (GenBank accession numbers indicated at left) for ORF3a and flanking regions. Spaces separate codons in the ORF3a reading frame. The alignment within ORF3a is based on a P3a amino acid alignment (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004868#ppat.1004868.s002" target="_blank">S1 Fig</a>). The 5' and 3' flanking sequences have not been aligned since pan-genus alignments in these regions are ambiguous. Virus name abbreviations: BLRV—<i>Bean leafroll virus</i>; SbDV—<i>Soybean dwarf virus</i>; CYDV-RPV—<i>Cereal yellow dwarf virus-RPV</i>; WYDV-GPV—<i>Wheat yellow dwarf virus-GPV</i>; CYDV-RPS—<i>Cereal yellow dwarf virus-RPS</i>; TuYV—<i>Turnip yellows virus</i>; BrYV—<i>Brassica yellows virus</i>; CpCSV—<i>Chickpea chlorotic stunt virus</i>; MABYV—<i>Melon aphid-borne yellows virus</i>; SABYV—<i>Suakwa aphid-borne yellows virus</i>; CLRDV—<i>Cotton leafroll dwarf virus</i>; ScYLV—<i>Sugarcane yellow leaf virus</i>; TVDV—<i>Tobacco vein distorting virus</i>; PeVYV—<i>Pepper vein yellows virus</i>; BWYV—<i>Beet western yellows virus</i>; BChV—<i>Beet chlorosis virus</i>; BMYV—<i>Beet mild yellowing virus</i>; CABYV—<i>Cucurbit aphid-borne yellows virus</i>; PLRV—<i>Potato leafroll virus</i>; CtRLV—<i>Carrot red leaf virus</i>; RSDaV—<i>Rose spring dwarf-associated virus</i>; MYDV-RMV—<i>Maize yellow dwarf virus-RMV</i>; BYDV-PAS—<i>Barley yellow dwarf virus-PAS</i>; BYDV-MAV—<i>Barley yellow dwarf virus-MAV</i>; BYDV-PAV—<i>Barley yellow dwarf virus-PAV</i>; BYDV-GAV—<i>Barley yellow dwarf virus-GAV</i>; BYDV-KerII—<i>Barley yellow dwarf virus Ker-II</i>.