Evaluation of integrins α4, αE, β1and β7 and CCR9 mRNA expression in WT BALB/c and IL-13 KO gut and splenic K^dGag_197–205-specific CD8⁺ T cells following i.n./i.m. vaccination.

<p>mRNA expression profiles in WT BALB/c and IL-13KO gut and splenic K^dGag_197–205-specific CD8⁺ T cells were evaluated using Fluidigm 48.48 dynamic arrays as described in methods. WT BALB/c spleen (SP) and Peyer’s patch (PP) n = 6 pools of hundred K^dGag_197–205-specific CD8⁺ T cells are indicated by blue circle and triangle respectively. IL-13 KO mice spleen n = 5 and Peyer’s patch n = 9 pools of hundred K^dGag_197–205-specific CD8⁺ T cells are indicated by red circles and triangles respectively. Data indicate log mRNA expression of integrin α4 (a) αE (b) β1 (c) β7 (d) and CCR9 (e) normalised to housekeeping gene L32. The experiment was repeated three times. Data represent the three experiments.