Evaluation of WT BALB/c and IL-13 KO gut and splenic K^dGag_{197–205 –}specific CD8⁺ α4β7⁺ and K^dGag_{197–205 –}specific CD8⁺ CCR9⁺ T cells following i.n./i.m. vaccination.

<p>WT BALB/c and IL-13 KO mice were prime boost immunized using i.n. FPV-HIV/i.m. VV-HIV strategy. Fourteen days post booster vaccination, splenic and gut K^dGag_197–205-specific CD8⁺ T cell responses were evaluated using K^dGag_197–205-tetramer staining together with α4β7 and CCR9 staining using FACS analysis. The percentage of WT BALB/c (blue) and IL-13 KO (red) splenic and gut K^dGag_197–205-tetramer⁺ CD8⁺ T cells (a and d), K^dGag_197–205-tetramer⁺ CD8⁺ α4β7⁺ T cells (b and e), K^dGag_197–205-tetramer⁺ CD8⁺ CCR9⁺ T cells (c and f) is shown. The percentage frequency of α4β7 and CCR9 homing marker expression on WT BALB/c and IL-13 KO splenic (g) and gut (h) K^dGag_197–205-tetramer⁺ CD8⁺ T cells is also shown. Data represent 4–5 experiments.