Immunocytochemistry of the <i>rd1</i>_Opto-mGluR6 retina.

<p>(A) Double labeling against cytoplasmic TurboFP635 (red) and ChAT (green), serving as depth marker in the inner plexiform layer (IPL), subdividing ON and OFF sublaminae (white brackets). All TurboFP635-positive bipolar cells project their axons to the ON-sublamina of the IPL. Nuclei are labeled with DAPI (<i>blue</i>). (B) Staining against the N-terminal part of melanopsin labels both ipRGCs and Opto-mGluR6 in the perikarya of ON-bipolar cells (ON-BPs). (C) Double labeling of TurboFP635 (red) with the pan ON-bipolar cell marker Gαo (green) shows a 100% overlay, indicating that all ON-BPs express Opto-mGluR6_IRES_TurboFP635. Scale bars, 20 μm.