Positional cloning of <i>ICA1</i>.

<p>(<i>A</i>) Whole genome scanning of F₂ (Sij-4 x Col-0) along with marker positions and recombination frequencies (R.f.) with <i>ICA1</i>. Map positions are given in Mb. (<i>B</i>) Fine mapping of <i>ICA1</i> in F₂ (Don-0 x L<i>er</i>-0), F₂ (Sij-4 x Col-0) and F₂ (Sij-4 x L<i>er</i>-0) segregating populations. Map positions and the number of recombinants are indicated above and below the line respectively. An overlapping interval of 5.9 kb contains a single gene. The single nucleotide deletion of Don-0 and the missense mutations between Col-0 and Sij-4 along with the corresponding amino acid changes are given below. (<i>C</i>) Phenotype of the Col-0 and <i>ica1-2</i> (Col-0 background) grown at 27°C. (D) Phenotype of <i>35S</i>::<i>amiR-ICA1</i> in Col-0 background at 23°C and 27°C. (<i>E</i>) Phenotypic complementation of Don-0 with an <i>ICA1</i>-L<i>er</i> transgene at 28°C (4 week-old plants). (<i>F</i>) Transgenic suppression of <i>ICA1</i>-Sij-4 phenotype at 27°C by <i>35S</i>::<i>ICA1gDNA-Col</i> and lack of phenotypic suppression with the S81P mutation.