TGF-β-mediated inhibition of naïve T cell proliferation is comparable between wildtype and <i>Drak2-/-</i> T cells.

<p>A) CD4⁺CD25^-CD44^lo naïve cells were purified from <i>OT-II</i> and <i>OT-II</i>.<i>Drak2-/-</i> mice and stimulated with irradiated splenocytes loaded with 10μM OVA₃₂₃ peptide in the presence or absence of 10-fold TGF-β titrations for three days. The number of live, divided Foxp3^-CD4⁺ cells are shown for each titration. Cells were obtained from one <i>OT-II</i> or <i>OT-II</i>.<i>Drak2-/-</i> mouse and tested in quadruplicate. Data are representative of five separate experiments. B) CD8⁺CD25^-CD44^loCD62L^hi naïve cells were purified from <i>OT-I</i> and <i>OT-I</i>.<i>Drak2-/-</i> mice and stimulated with splenocytes loaded with 100pM OVA₂₅₇ peptide in the presence or absence of 10-fold TGF-β titrations. Two days later, cells were harvested and analyzed by flow cytometry. The number of live, divided CD8⁺ cells are shown for each titration. Cells were obtained from one <i>OT-I</i> or <i>OT-I</i>.<i>Drak2-/-</i> mouse and tested in quadruplicate. Data are representative of three separate experiments. There was no significant difference in the response of the wildtype and <i>Drak2-/-</i> cells according to the Mann-Whitney <i>U</i>-test.