Kinetics of pulmonary cytokine expression after <i>M</i>. <i>tuberculosis</i> infection.

<p>Total RNA was prepared from the lungs of naïve WT (black symbols) and TNF-α deficient mice (grey symbols) and at days 7, 14, 21 and 27 after <i>M</i>. <i>tuberculosis</i> infection in WT (black symbols) and at day 27 in TNF-α deficient mice (grey symbols). The results represent the levels of <i>Il36g</i>, <i>Il36r</i>, <i>Tnfa</i>, <i>Il1b</i>, and <i>Ifng</i> mRNA determined by RT-qPCR normalized to <i>L32</i> mRNA (n = 5 per group). *P<0.05, **P<0.01, as assessed by Kruskal-Wallis, followed by Mann-Whitney test (C).