Rab27A deficiency caused increased expression of Rab27B, but did not affect its activity.

<p>(<b>A</b> and <b>B</b>) Examples of total lysates of isolated pancreatic acinar cells from wild-type C3H/HeSnJ or <i>ashen</i> mice were analyzed by western blot. Each lane represents samples from one mouse. (<b>B</b>) Densitometry analysis on the western blot results from all samples run as in (A). The results are mean ± SE from five mice of each genotype. *P < 0.05. (<b>C</b>) Active form of Rab27B and Rab3D at basal level in isolated acini was examined by GST-SHD and GST-Rim pulldown, respectively. Pulldown fractions were analyzed by western blot. This experiment was repeated three times with similar results. (<b>D</b>) The expression of major digestive enzymes (amylase, chymotrypsin, lipase, and elastase) and other Rab proteins (Rab6 and Rab11) was also not changed in western blots on lysates from isolated <i>ashen</i> mouse acinar cells. (<b>E</b>) The expression of major digestive enzymes (amylase, chymotrypsin, elastase, carboxypeptidase A and ribonuclease A) was also not changed in western blots of purified <i>ashen</i> mouse zymogen granules.