Effect of pregnancy on neuronal NO synthesis.

<p>(A) Effect of 0.1 mmo/L L-NAME or 0.1 mmol/L 7-NI or 0.1 μmol/L TTX on EFS-induced NO release in segments from control and pregnant rats. Results (mean±SEM) were expressed as arbitrary (A.U.)/mg tissue. *P<0.05 vs. Control; #P<0.05 compared with conditions without specific inhibitor; n = 8 animals per group. (B) Effect of pregnancy on nNOS and P-nNOS expression. The blot is representative of four separate segments from each group. Rat brain homogenates were used as a positive control. Lower panel shows relation between P-nNOS or nNOS expression and β-actin. Results (mean±SEM) are expressed as ratio of the signal obtained for each protein and the signal obtained for β-actin. *P<0.05 Control vs. pregnant rats.